Chemiluminescent Substances

Chemiluminescent and bioluminescent techniques.

Light-emitting chemical reactions (chemiluminescence, CL) and biological reactions (bioluminescence, BL) have a diverse range of analytical applications but relatively few have been adopted by routine clinical laboratories. Advantages of CL and BL assays include sensitivity (attomole and sub-attomole detection limits), speed (signal generated in a few seconds and in some cases stable for severa...

A homogeneous chemiluminescent immunoassay method.

A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proximity. Without any separation steps, a chemiluminescent signal is generated upon addition of a trig...

Electrogenerated chemiluminescent determination of oxalate.

Chemiluminescent Immunoassay and Its Applications

Chemiluminescent immunoassay (CLIA) has been applied in different fields, including environmental monitoring, clinical diagnosis, food safety and pharmaceutical analysis, as a promising approach for selective, sensitive, rapid and simple analysis. It is often necessary to detect a large number of complicated or low-abundance samples. However, traditional methods need great consumption of time, ...

Development of chemiluminescent methods for explosives detection

The present work was finalised to develop chemiluminescent assays more sensitive and easy to use than the analytical methods currently in use to detect traces of TNT (2,4,6-trinitrotoluene), TATP (Triacetone triperoxide) and HMTD (Hexamethylene triperoxide diamine). For TNT detection we developed an indirect competitive ELISA with chemiluminescent end-point (CL-ELISA) and a Lateral Flow ImmunoA...